Diminished Ciprofloxacin-Induced Chondrotoxicity by Supplementation of Magnesium and Vitamin E in Immature Rats Running Title Ciprofloxacin-induced Chondrotoxicity

Quinolone-induced chondrotoxicity was demonstrated in juvenile rats and multiple other species. Identical damages can be induced in immature rats by feeding a magnesium-deficient diet. The objective of the present study was to investigate whether in reverse the oral supplementation with magnesium, vitamin E or both can diminish the typical quinoloneinduced arthropathy in juvenile Wistar rats. Four groups of 12 (6 male, 6 female) 24-day-old Wistar rats each were fed for 10 days with either normal feed (A), vitamin E enriched diet (B), magnesium enriched diet (C) or vitamin E/magnesium-enriched diet (D). All rats received two s.c. ciprofloxacin doses of 600 mg/kg on day 32 postnatally. Two days later, rats were sacrificed and cartilage samples of knee joint were examined under a light microscope for presence of typically quinolone-induced joint cartilage lesions. In addition, magnesium, calcium and vitamin E concentrations in cartilage and plasma were determined. In the rats under normal diet (A), 17 quinolone-induced joint cartilage lesions were observed. In groups with enriched diet the incidence of specific lesions (n) was significantly lower (% reduction compared to group A): B, n=10 (41%, p<0.05); C, n=6 (65%, p<0.01); and D, n=3 (82 %, p<0.01). In comparison to standard diet, magnesium and vitamin E supplementation resulted in significantly higher magnesium and vitamin E concentrations in plasma and articular cartilage, respectively. In conclusion, the supplementation of magnesium and vitamin E alone or in combination may relevantly diminish joint cartilage lesions induced by quinolones in immature rats with an additive effect of the combined supplementation. The data further support the proposed AC CE PT ED on N ovem er 3, 2017 by gest httpaac.asm .rg/ D ow nladed fom pathomechanism of quinolone induced arthropathy and the crucial role of magnesium in immature joint cartilage.